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5–four μmol/L assortment,[156][157] the focus of serotonin in synaptic vesicles was calculated at 270 mM.[182] Following vesicular launch, the resulting concentration of serotonin from the synaptic cleft, to which serotonin receptors are exposed, is believed for being about 300 μmol/L. Therefore, when in vitro receptor binding affinities, eff